Bacterial Growth at Constant pH

Maintained at Constant Ph by Walther

Bacterial growth: constant obsession with dN/dt.

Assay of carbonic anhydrase by titration at constant pH.

1. A method is described for measuring accurately the initial velocity of the hydration reaction catalysed by the enzyme carbonic anhydrase (EC 4.2.1.1); the method depends on the titration of H(+) ions at constant pH. 2. Human erythrocyte carbonic anhydrase, isoenzyme C, was used to illustrate the method. Under the experimental conditions employed (0 degrees , pH7.0, in the presence of 45mm-so...

GUEST COMMENTARY Bacterial Growth: Constant Obsession with dN/dt

One of life’s inevitable disappointments—one felt often by scientists and artists, but not only by them—comes from expecting others to share the particularities of one’s own sense of awe and wonder. This truth came home to me recently when I picked up Michael Guillen’s fine book Five Equations That Changed the World (4) and discovered that my equation—the one that shaped my scientific career—wa...

High-performance molecular dynamics at constant pH and constant redox potential using AMBER

Protein function is intrinsically related to structure and dynamic. Solution pH often has a dramatic impact on protein systems, since pH can affect the charge distribution within these biomolecules.[1] Understanding how pH-dependent conformation changes take place is key to the development of drugs targeting pH sensors, to the success of computational drug discovery strategies, and so forth.[2]...